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cometchip raw data and analyzed results, part six  (Mendeley Ltd)

 
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    Mendeley Ltd cometchip raw data and analyzed results, part six
    Optimization of <t>CometChip</t> (A and B) Representative pictures of “comet” of ring and schizont-stage P. falciparum 3D7 after on-chip hydrogen peroxide (H 2 O 2 ) treatment. Each green dot indicates a microwell in the CometChip. Each macrowell of the 96-well plate contains around ~300 of such microwells. The magnified view of the boxed area below shows the differentiation of head (between the green line and red line) and tail (between the red line and blue line) of an individual comet. This process would be automatically done by the algorithm as shown in the . Scale bars, 50 nm. (C) Quantification of “comet” after treatment. The y axis represents the percentage of DNA at the “comet” tail (%Tail DNA). On average, ~306 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates. ∗ p < 0.05; ∗∗ p < 0.01, one-way analysis of variance (ANOVA). Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.
    Cometchip Raw Data And Analyzed Results, Part Six, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cometchip raw data and analyzed results, part six/product/Mendeley Ltd
    Average 90 stars, based on 1 article reviews
    cometchip raw data and analyzed results, part six - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "K13-Mediated Reduced Susceptibility to Artemisinin in Plasmodium falciparum Is Overlaid on a Trait of Enhanced DNA Damage Repair"

    Article Title: K13-Mediated Reduced Susceptibility to Artemisinin in Plasmodium falciparum Is Overlaid on a Trait of Enhanced DNA Damage Repair

    Journal: Cell Reports

    doi: 10.1016/j.celrep.2020.107996

    Optimization of CometChip (A and B) Representative pictures of “comet” of ring and schizont-stage P. falciparum 3D7 after on-chip hydrogen peroxide (H 2 O 2 ) treatment. Each green dot indicates a microwell in the CometChip. Each macrowell of the 96-well plate contains around ~300 of such microwells. The magnified view of the boxed area below shows the differentiation of head (between the green line and red line) and tail (between the red line and blue line) of an individual comet. This process would be automatically done by the algorithm as shown in the . Scale bars, 50 nm. (C) Quantification of “comet” after treatment. The y axis represents the percentage of DNA at the “comet” tail (%Tail DNA). On average, ~306 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates. ∗ p < 0.05; ∗∗ p < 0.01, one-way analysis of variance (ANOVA). Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.
    Figure Legend Snippet: Optimization of CometChip (A and B) Representative pictures of “comet” of ring and schizont-stage P. falciparum 3D7 after on-chip hydrogen peroxide (H 2 O 2 ) treatment. Each green dot indicates a microwell in the CometChip. Each macrowell of the 96-well plate contains around ~300 of such microwells. The magnified view of the boxed area below shows the differentiation of head (between the green line and red line) and tail (between the red line and blue line) of an individual comet. This process would be automatically done by the algorithm as shown in the . Scale bars, 50 nm. (C) Quantification of “comet” after treatment. The y axis represents the percentage of DNA at the “comet” tail (%Tail DNA). On average, ~306 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates. ∗ p < 0.05; ∗∗ p < 0.01, one-way analysis of variance (ANOVA). Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.

    Techniques Used: Comparison, Control

    Artesunate-Induced DNA Damage in P. falciparum Lab Strains (A) Schematic illustration of artesunate treatment based on Streptolysin O (SLO) enrichment (ring stage) or Percoll gradient centrifugation (schizont stage). (B) Artesunate-induced DNA damage in P. falciparum 3D7, Dd2, and Dd2 I543T . Ring-stage (upper panel) and schizont-stage (lower panel) P. falciparum parasites were treated with different concentrations of artesunate for 1 h and subjected to analysis on alkaline CometChip. On average, ~302 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates or quadruplicates. ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001, one-way ANOVA. Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.
    Figure Legend Snippet: Artesunate-Induced DNA Damage in P. falciparum Lab Strains (A) Schematic illustration of artesunate treatment based on Streptolysin O (SLO) enrichment (ring stage) or Percoll gradient centrifugation (schizont stage). (B) Artesunate-induced DNA damage in P. falciparum 3D7, Dd2, and Dd2 I543T . Ring-stage (upper panel) and schizont-stage (lower panel) P. falciparum parasites were treated with different concentrations of artesunate for 1 h and subjected to analysis on alkaline CometChip. On average, ~302 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates or quadruplicates. ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001, one-way ANOVA. Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.

    Techniques Used: Gradient Centrifugation, Comparison, Control


    Figure Legend Snippet:

    Techniques Used: Recombinant, Giemsa Stain, Saline, Purification, Plasmid Preparation, Software



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    cometchip raw data and analyzed results, part six  (Mendeley Ltd)
    90
    Mendeley Ltd cometchip raw data and analyzed results, part six
    Optimization of <t>CometChip</t> (A and B) Representative pictures of “comet” of ring and schizont-stage P. falciparum 3D7 after on-chip hydrogen peroxide (H 2 O 2 ) treatment. Each green dot indicates a microwell in the CometChip. Each macrowell of the 96-well plate contains around ~300 of such microwells. The magnified view of the boxed area below shows the differentiation of head (between the green line and red line) and tail (between the red line and blue line) of an individual comet. This process would be automatically done by the algorithm as shown in the . Scale bars, 50 nm. (C) Quantification of “comet” after treatment. The y axis represents the percentage of DNA at the “comet” tail (%Tail DNA). On average, ~306 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates. ∗ p < 0.05; ∗∗ p < 0.01, one-way analysis of variance (ANOVA). Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.
    Cometchip Raw Data And Analyzed Results, Part Six, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cometchip raw data and analyzed results, part six/product/Mendeley Ltd
    Average 90 stars, based on 1 article reviews
    cometchip raw data and analyzed results, part six - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Optimization of CometChip (A and B) Representative pictures of “comet” of ring and schizont-stage P. falciparum 3D7 after on-chip hydrogen peroxide (H 2 O 2 ) treatment. Each green dot indicates a microwell in the CometChip. Each macrowell of the 96-well plate contains around ~300 of such microwells. The magnified view of the boxed area below shows the differentiation of head (between the green line and red line) and tail (between the red line and blue line) of an individual comet. This process would be automatically done by the algorithm as shown in the . Scale bars, 50 nm. (C) Quantification of “comet” after treatment. The y axis represents the percentage of DNA at the “comet” tail (%Tail DNA). On average, ~306 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates. ∗ p < 0.05; ∗∗ p < 0.01, one-way analysis of variance (ANOVA). Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.

    Journal: Cell Reports

    Article Title: K13-Mediated Reduced Susceptibility to Artemisinin in Plasmodium falciparum Is Overlaid on a Trait of Enhanced DNA Damage Repair

    doi: 10.1016/j.celrep.2020.107996

    Figure Lengend Snippet: Optimization of CometChip (A and B) Representative pictures of “comet” of ring and schizont-stage P. falciparum 3D7 after on-chip hydrogen peroxide (H 2 O 2 ) treatment. Each green dot indicates a microwell in the CometChip. Each macrowell of the 96-well plate contains around ~300 of such microwells. The magnified view of the boxed area below shows the differentiation of head (between the green line and red line) and tail (between the red line and blue line) of an individual comet. This process would be automatically done by the algorithm as shown in the . Scale bars, 50 nm. (C) Quantification of “comet” after treatment. The y axis represents the percentage of DNA at the “comet” tail (%Tail DNA). On average, ~306 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates. ∗ p < 0.05; ∗∗ p < 0.01, one-way analysis of variance (ANOVA). Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.

    Article Snippet: CometChip raw data and analyzed results, Part six , This paper; Mendeley data , https://doi.org/10.17632/grdkg7rb83.1.

    Techniques: Comparison, Control

    Artesunate-Induced DNA Damage in P. falciparum Lab Strains (A) Schematic illustration of artesunate treatment based on Streptolysin O (SLO) enrichment (ring stage) or Percoll gradient centrifugation (schizont stage). (B) Artesunate-induced DNA damage in P. falciparum 3D7, Dd2, and Dd2 I543T . Ring-stage (upper panel) and schizont-stage (lower panel) P. falciparum parasites were treated with different concentrations of artesunate for 1 h and subjected to analysis on alkaline CometChip. On average, ~302 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates or quadruplicates. ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001, one-way ANOVA. Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.

    Journal: Cell Reports

    Article Title: K13-Mediated Reduced Susceptibility to Artemisinin in Plasmodium falciparum Is Overlaid on a Trait of Enhanced DNA Damage Repair

    doi: 10.1016/j.celrep.2020.107996

    Figure Lengend Snippet: Artesunate-Induced DNA Damage in P. falciparum Lab Strains (A) Schematic illustration of artesunate treatment based on Streptolysin O (SLO) enrichment (ring stage) or Percoll gradient centrifugation (schizont stage). (B) Artesunate-induced DNA damage in P. falciparum 3D7, Dd2, and Dd2 I543T . Ring-stage (upper panel) and schizont-stage (lower panel) P. falciparum parasites were treated with different concentrations of artesunate for 1 h and subjected to analysis on alkaline CometChip. On average, ~302 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates or quadruplicates. ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001, one-way ANOVA. Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.

    Article Snippet: CometChip raw data and analyzed results, Part six , This paper; Mendeley data , https://doi.org/10.17632/grdkg7rb83.1.

    Techniques: Gradient Centrifugation, Comparison, Control

    Journal: Cell Reports

    Article Title: K13-Mediated Reduced Susceptibility to Artemisinin in Plasmodium falciparum Is Overlaid on a Trait of Enhanced DNA Damage Repair

    doi: 10.1016/j.celrep.2020.107996

    Figure Lengend Snippet:

    Article Snippet: CometChip raw data and analyzed results, Part six , This paper; Mendeley data , https://doi.org/10.17632/grdkg7rb83.1.

    Techniques: Recombinant, Giemsa Stain, Saline, Purification, Plasmid Preparation, Software